Anti-14-3-3 Sigma / Stratifin Monoclonal Antibody (Clone: CPTC-SFN-2)

SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Product Specifications

Product Name Alternative

SFN; HME1; Stratifin; YWHAS; 14-3-3 Sigma

Gene Name

SFN

Gene ID

2810

UniProt

P31947

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length SFN protein

Clonality

Monoclonal

Clone

CPTC-SFN-2

Applications

WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)