Anti-GAD2 / GAD65 (GABAergic Neuronal Marker) Monoclonal Antibody (Clone: GAD2/2362)

This MAb recognizes a protein of 65kDa, which is identified as glutamic acid decarboxylase 2 (GDA2) . It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD's) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67) . GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Althoμgh both GAD's are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throμghout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.

Product Specifications

Product Name Alternative

DCE2; GAD2; Glutamic Acid Decarboxylase 2 (Pancreas) ; Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65kDa) ; Glutamic Acid Decarboxylase 65 (GAD65)

Gene Name

GAD2

Gene ID

2572

UniProt

Q05329

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment of human GAD2 (GAD65) protein (around aa 6-99) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

GAD2/2362

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)