Anti-Blood Group Antigen Lewis B Monoclonal Antibody (Clone: 2-25LE; same as LWB01)

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha (1,3) -fucosyltransferase and alpha (1,4) -fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.

Product Specifications

Product Name Alternative

Blood group Lewis alpha-4-fucosyltransferase; Fucosyltransferase 3; Fucosyltransferase III; FucT-III; FUT3; Galactoside 3 (4) -L-fucosyltransferase; gastric mucin; leB; lewis antigen system; lewis B blood group antigen; Lewis FT; major airway glycoprotein

Gene Name

FUT3

Gene ID

2525

UniProt

P21217

Host

Mouse

Reactivity

Human

Immunogen

Mucin isolated from a human ovarian cyst fluid

Clonality

Monoclonal

Clone

2-25LE; same as LWB01

Applications

IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)