Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (Clone: B1D8)

This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF-alphainduced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-alphaa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.

Product Specifications

Product Name Alternative

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Gene Name

EGFR

Gene ID

1956

UniProt

P00533

Host

Mouse

Reactivity

Human

Immunogen

Microsomes from A431 cells.

Clonality

Monoclonal

Clone

B1D8

Applications

IP, FACS

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Affinity Purification (For coupling, order Ab without BSA) ; Blocks Ligand-induced Activation of EGFR; Inhibits Proliferation of A431 cells (Order Ab without sodium azide) ; Arrests Tumor Growth in vivo (Order Ab without BSA &Azide) ; Immunoprecipitation (1-2ug/500ug protein lysate) ; Flow Cytometry (1-2ug/million cells)