Anti-Secretory Component / ECM1 Monoclonal Antibody (Clone: SC05)

This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Product Specifications

Product Name Alternative

ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD

Gene Name

ECM1

Gene ID

1893

UniProt

Q16610

Host

Mouse

Reactivity

Human

Immunogen

Secretory Component protein isolated from human colostrum

Clonality

Monoclonal

Clone

SC05

Applications

FACS, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (0.5-1ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)