Anti-DNMT1 / DNA Methyltransferase 1 Monoclonal Antibody (Clone: DNMT1/2061)

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE.

Product Specifications

Product Name Alternative

ADCADN; AIM; CXXC-type zinc finger protein 9; CXXC9; DNA methyltransferase 1 (DNMT1) ; HSN1E; MCMT; Met1; MommeD2

Gene Name

DNMT1

Gene ID

1786

UniProt

P26358

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human DNMT1 protein fragment (around aa 767-912) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

DNMT1/2061

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)