Anti-Drebrin 1 (DBN1) Monoclonal Antibody (Clone: DBN1/3393)

Drebrins (developmentally regulated brain proteins) are cytoplasmic proteins that bind F-actin in the brain and are involved in cell migration, extension of neuronal processes and plasticity of dendrites. There are three isoforms: two embryonic types (E1 and E2) ; and an adult type (A), generated by alternative RNA splicing from a single Drebrin gene. Drebrins are expressed mainly in brain neurons but are also found in skeletal muscle, heart, placenta, pancreas and kidney. Drebrin has been designated as a marker of the dendritic spine. Decreases in Drebrin levels in the brain have been been associated with Alzheimer s disease and Down syndrome.

Product Specifications

Product Name Alternative

Developmentally regulated brain protein; Drebrin 1; Drebrin; Drebrin E; Drebrin E2; Drebrin1; DrebrinE

Gene Name

DBN1

Gene ID

1627

UniProt

Q16643

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (aa 150-281) of human DBN1 protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

DBN1/3393

Applications

IHC, WB

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)