Anti-Catenin, beta (p120) Monoclonal Antibody (Clone: 6F9)

This MAb recognizes a protein of 92kDa, which is identified as beta-catenin. It shows no cross-reaction with gamma-catenin (also known as plakoglobin) . The catenins, alpha, beta and gamma bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Alpha-catenin was initially described as an E-cadherin-associated protein, and has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin has also been found in complexes with the tumor suppressor protein APC.

Product Specifications

Product Name Alternative

Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Gene Name

CTNNB1

Gene ID

1499

UniProt

P35222

Host

Mouse

Reactivity

Human

Immunogen

Recombinant chicken Î2-catenin

Clonality

Monoclonal

Clone

6F9

Applications

FACS, IF, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)