Anti-p21WAF1 (Tumor Suppressor Protein) Monoclonal Antibody (Clone: AC8)

This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma) .

Product Specifications

Product Name Alternative

Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, p21Cip1/Waf1, PIC1, SDI1, SLC12A9, Wild type p53 activated fragment 1 (WAF1)

Gene Name

CDKN1A

Gene ID

1026

UniProt

P38936

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CDKN1A protein

Clonality

Monoclonal

Clone

AC8

Applications

ELISA, WB, IP, IF

Components

200μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Applications Notes

ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA) ; Western Blot (1-2ug/ml) ; Immunoprecipitation (1-2ug/500ug protein) ; Immunofluorescence (1-2ug/ml)