Anti-Occludin (OCLN) (Tight Junctions Marker) Monoclonal Antibody (Clone:OCLN/2183)

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

Product Specifications

Product Name Alternative

BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PPP1R115; PTORCH1; Tight junction protein occludin

Gene Name

OCLN

Gene ID

100506658

UniProt

Q16625

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Occludin fragment around aa 282-415 (Exact sequence is proprietary)

Clonality

Monoclonal

Clone

OCLN/2183

Applications

IHC

Components

200μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)