Mouse Monoclonal Antibody To N-Cadherin (Clone: 13A9)
Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Product Specifications
Gene Name
CDH2
Gene ID
1000
UniProt
P19022
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Recombinant human N-cadherin cytoplasmic domain (exact sequence is proprietary)
Target Antigen
N-Cadherin
Clonality
Monoclonal
Clone
13A9
Applications
FACS, IF, WB
Purification
Affinity Chromatography
Format
Purified
Components
200 μg/mL of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Storage Conditions
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Flow Cytometry (1-2ug/million cells) ; , Immunofluorescence (1-2ug/ml) ; , Western Blotting (1-2ug/ml) ;
Isotype
Mouse IgG1, kappa
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