Monoclonal Antibody to Cdk1 / p34cdc2 (Clone : POH-1; same as cdc2.1)

Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Product Specifications

Product Name Alternative

CDK1||CDC2||CDC28A||CDKN1||P34CDC2

Gene Name

CDK1

Gene ID

983

UniProt

P06493

Host

Mouse

Reactivity

Human

Immunogen

Purified recombinant human p34cdc2 fusion protein

Target Antigen

Cdk1 / p34cdc2

Clonality

Monoclonal

Clone

POH-1; same as cdc2.1

Applications

WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)