Monoclonal Antibody to CD59 / Complement Regulatory Protein / Protectin (Clone : 193-27)
Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI) -anchored cell surface protein (Workshop VI; Code N-L036) . CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH) . This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.
Product Specifications
Product Name Alternative
CD59||MIC11||MIN1||MIN2||MIN3||MSK21
Gene Name
CD59
Gene ID
966
UniProt
P13987
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes
Target Antigen
CD59 / Complement Regulatory Protein / Protectin
Clonality
Monoclonal
Clone
193-27
Applications
Functional Assay, FACS, IF
Purification
Affinity Chromatography
Format
Purified
Components
100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Functional Studies (Order Ab without Azide) ; , Flow Cytometry (1-2ug/million cells) ; , Immunofluorescence (1-2ug/ml) ;
Isotype
Mouse IgM, kappa
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