Monoclonal Antibody to CD34 (Hematopoietic Stem Cell & Endothelial Marker) (Clone : SPM610)

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens) . Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

Product Specifications

Product Name Alternative

CD34

Gene Name

CD34

Gene ID

947

UniProt

P28906

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant full-length human HPCA1 protein

Target Antigen

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Clonality

Monoclonal

Clone

SPM610

Applications

FACS, IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; , Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)