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Polyclonal Antibody to p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker)

P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Product Specifications

Product Name Alternative

TP63||KET||P63||P73H||P73L||TP73L

Gene Name

TP63

Gene ID

8626

UniProt

Q9H3D4

Host

Rabbit

Reactivity

Rat, Mouse, Human

Immunogen

A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Target Antigen

P40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker)

Clonality

Polyclonal

Applications

WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Isotype

Rabbit IgG

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