Monoclonal Antibody to Carbonic Anhydrase IX (Renal Cell Marker) (Clone : CA9/781)

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200) . Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.

Product Specifications

Product Name Alternative

CA9||G250||MN

Gene Name

CA9

Gene ID

768

UniProt

Q16790

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CA9 protein

Target Antigen

Carbonic Anhydrase IX (Renal Cell Marker)

Clonality

Monoclonal

Clone

CA9/781

Applications

WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)