Monoclonal Antibody to VEGF (Vascular Endothelial Growth Factor) (Clone : SPM225)

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Product Specifications

Product Name Alternative

VEGFA||VEGF

Gene Name

VEGFA

Gene ID

7422

UniProt

P15692

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Human VEGF189 recombinant protein

Target Antigen

VEGF (Vascular Endothelial Growth Factor)

Clonality

Monoclonal

Clone

SPM225

Applications

IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)