Monoclonal Antibody to GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) (Clone : SPM249)

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96) . Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90) . This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Product Specifications

Product Name Alternative

HSP90B1||GRP94||TRA1

Gene Name

HSP90B1

Gene ID

7184

UniProt

P14625

Host

Rat

Reactivity

Human, Rat, Mouse, Hamster

Immunogen

Purified glucose regulated protein 94 (grp94) from chicken oviducts

Target Antigen

GRP94 / HSP90B1 (Endoplasmic Reticulum Marker)

Clonality

Monoclonal

Clone

SPM249

Applications

FACS, IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)