Monoclonal Antibody to TNF-alphalpha (Tumor Necrosis Factor alpha) (TNF706 + P/T2)

TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Product Specifications

Product Name Alternative

TNF||TNFA||TNFSF2

Gene Name

TNF

Gene ID

7124

UniProt

P01375

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant N-terminal fragment of human TNF-alpha (TNF706) ; Peptide corresponding to aa115-130 of human TNF-alpha (P/T2)

Target Antigen

TNF-alphalpha (Tumor Necrosis Factor alpha)

Clonality

Monoclonal

Clone

TNF706 + P/T2

Applications

IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)