Monoclonal Antibody to TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) (Clone : NX2.1/690)

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1) . TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Product Specifications

Product Name Alternative

NKX2-1||NKX2A||TITF1||TTF1

Gene Name

NKX2-1

Gene ID

7080

UniProt

P43699

Host

Mouse

Reactivity

Human

Immunogen

Recombinant TTF-1 protein

Target Antigen

TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker)

Clonality

Monoclonal

Clone

NX2.1/690

Applications

IHC, FACS, IF

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)