Monoclonal Antibody to TGF-alphaalpha (Transforming Growth Factor alpha) (Clone : SPM542)

This antibody reacts with the TGF-alphaalpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-alpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Product Specifications

Product Name Alternative

TGF-alpha

Gene Name

TGF-alpha

Gene ID

7039

UniProt

P01135

Host

Mouse

Reactivity

Human

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF-alphaAlpha.

Target Antigen

TGF-alphaalpha (Transforming Growth Factor alpha)

Clonality

Monoclonal

Clone

SPM542

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)