Monoclonal Antibody to CD147 / EMMPRIN / Neurothelin (Clone : 8D6)

This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Product Specifications

Product Name Alternative

BSG||UNQ6505/PRO21383

Gene Name

BSG

Gene ID

682

UniProt

P35613

Host

Mouse

Reactivity

Human

Immunogen

Human T cell line Molt 13

Target Antigen

CD147 / EMMPRIN / Neurothelin

Clonality

Monoclonal

Clone

8D6

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, & Migation through Matrigel (Order w/o BSA / azide) ; , Flow Cytometry (0.5-1ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)