Monoclonal Antibody to CD43 (T-Cell Marker) (Clone : Bra7G)

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V) . Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Product Specifications

Product Name Alternative

SPN, CD43

Gene Name

SPN

Gene ID

6693

UniProt

P16150

Host

Mouse

Reactivity

Human

Immunogen

Immature pluripotent human leukemia K562 cells

Target Antigen

CD43 (T-Cell Marker)

Clonality

Monoclonal

Clone

Bra7G

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at room temp)