Monoclonal Antibody to Calgranulin B (Macrophage Marker) (Clone : CAGB/426)

Recognizes a 12-14kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14) ; expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Product Specifications

Product Name Alternative

S100A9||CAGB||CFAG||MRP14

Gene Name

S100A9

Gene ID

6280

UniProt

P06702

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CAGB protein

Target Antigen

Calgranulin B (Macrophage Marker)

Clonality

Monoclonal

Clone

CAGB/426

Applications

IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)