Monoclonal Antibody to Retinol Binding Protein-1 (RBP1) (Clone : SPM442)

Recognizes a protein of 21kDa-25kDa, identified as retinol binding protein (RBP) . Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin) . RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.

Product Specifications

Product Name Alternative

RBP4||PRO2222

Gene Name

RBP4

Gene ID

5950

UniProt

P02753

Host

Mouse

Reactivity

Human, Rat, Mouse

Immunogen

Retinol binding protein purified from human plasma

Target Antigen

Retinol Binding Protein-1 (RBP1)

Clonality

Monoclonal

Clone

SPM442

Applications

IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)