Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker) (Clone : E29)

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

Product Name Alternative

MUC1||PUM

Gene Name

MUC1

Gene ID

4582

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Delipidated extract of human milk fat globule membranes

Target Antigen

MUC1 / EMA / CD227 (Epithelial Marker)

Clonality

Monoclonal

Clone

E29

Applications

IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)