Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker) (Clone : GP1.4)

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

Product Name Alternative

MUC1||PUM

Gene Name

MUC1

Gene ID

4582

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Human milk fat globule membranes

Target Antigen

MUC1 / EMA / CD227 (Epithelial Marker)

Clonality

Monoclonal

Clone

GP1.4

Applications

WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2ug/ml) ; , Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)