Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker) (Clone : HMPV)

This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.

Product Specifications

Product Name Alternative

MUC1||PUM

Gene Name

MUC1

Gene ID

4582

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Human breast cancer cell line ZR-75 cells

Target Antigen

MUC1 / EMA / CD227 (Epithelial Marker)

Clonality

Monoclonal

Clone

HMPV

Applications

WB, FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2ug/ml) ; Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunohistology (Formalin-paraffin) (0.5-1.0ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)