Monoclonal Antibody to Milk Fat Globule (Breast Epithelial Marker) (Clone : EDM45)

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG) . HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Product Specifications

Product Name Alternative

MFGE8

Gene Name

MFGE8

Gene ID

4240

UniProt

Q08431

Host

Mouse

Reactivity

Human

Immunogen

Delipidated human milk fat globule membrane prepaRation

Target Antigen

Milk Fat Globule (Breast Epithelial Marker)

Clonality

Monoclonal

Clone

EDM45

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)