Monoclonal Antibody to MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) (Clone : C146/634)

The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Specifications

Product Name Alternative

MCAM||MUC18

Gene Name

MCAM

Gene ID

4162

UniProt

P43121

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CD146 protein

Target Antigen

MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule)

Clonality

Monoclonal

Clone

C146/634

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95&degC followed by cooling at RT for 20 minutes)