Monoclonal Antibody to MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) (Clone : SPM620)

The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Specifications

Product Name Alternative

MCAM||MUC18

Gene Name

MCAM

Gene ID

4162

UniProt

P43121

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human MCAM protein

Target Antigen

MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule)

Clonality

Monoclonal

Clone

SPM620

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)