Monoclonal Antibody to Ep-CAM / CD326 (Epithelial Marker) (Clone : SPM528)

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

Product Name Alternative

EPCAM||GA733-2||M1S2||M4S1||MIC18||TACSTD1||TROP1

Gene Name

EPCAM

Gene ID

4072

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

Neuraminidase treated GLS-1 human small cell lung carcinoma cells

Target Antigen

Ep-CAM / CD326 (Epithelial Marker)

Clonality

Monoclonal

Clone

SPM528

Applications

FACS, IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (5-10ul/million cells) ; Immunofluorescence (1:50-1:100) ; Western Blot (1:100-1:200) ; Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 min at RT) (Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)