Monoclonal Antibody to Prostate Specific Antigen (PSA) (KLK3/801 + KLK3/1248)

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA) . This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT) . A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Product Specifications

Product Name Alternative

KLK3||APS

Gene Name

KLK3

Gene ID

354

UniProt

P07288

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human KLK3 protein

Target Antigen

Prostate Specific Antigen (PSA)

Clonality

Monoclonal

Clone

KLK3/801 + KLK3/1248

Applications

IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)