Monoclonal Antibody to HSP27 (Heat Shock Protein 27) (Clone : HSPB1/774)

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Product Specifications

Product Name Alternative

HSPB1||HSP27||HSP28

Gene Name

HSPB1

Gene ID

3315

UniProt

P04792

Host

Mouse

Reactivity

Human, Rat, Mouse

Immunogen

Recombinant human HSPB1 protein

Target Antigen

HSP27 (Heat Shock Protein 27)

Clonality

Monoclonal

Clone

HSPB1/774

Applications

FACS, IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (0.25-0.5ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)