Monoclonal Antibody to HSP27 (Heat Shock Protein 27) (Clone : SPM252)

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Product Specifications

Product Name Alternative

HSPB1||HSP27||HSP28

Gene Name

HSPB1

Gene ID

3315

UniProt

P04792

Host

Mouse

Reactivity

Human, Rat, Mouse

Immunogen

Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Target Antigen

HSP27 (Heat Shock Protein 27)

Clonality

Monoclonal

Clone

SPM252

Applications

FACS, IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)