Monoclonal Antibody to Ku (p70/p80) (Nuclear Marker) (Clone : KU729)

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Product Specifications

Product Name Alternative

XRCC6||G22P1

Gene Name

XRCC6

Gene ID

2547

UniProt

P12956

Host

Mouse

Reactivity

Human

Immunogen

Nuclear extract of human HL-60 cells

Target Antigen

Ku (p70/p80) (Nuclear Marker)

Clonality

Monoclonal

Clone

KU729

Applications

FACS, IF, ICC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunocytochemistry (Acetone-fixed cells) (0.5-1.0ug/ml for 30 minutes at RT)