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Monoclonal Antibody to CD15 / FUT4 (Reed-Sternberg Cell Marker) (Clone : Leu-M1; same as MMA)

Selected Variant

36-1161-100

Size: 100 µg

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Product Specifications

Product Name Alternative

FUT4||ELFT||FCT3A

Gene Name

FUT4

Gene ID

2526

UniProt

P22083

Host

Mouse

Reactivity

Human

Immunogen

U937 histiocytic cell line

Target Antigen

CD15 / FUT4 (Reed-Sternberg Cell Marker)

Clonality

Monoclonal

Clone

Leu-M1; same as MMA

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Isotype

Mouse IgM, kappa

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