Monoclonal Antibody to TRIM29 (Lung Squamous Cell Carcinoma Marker) (Clone : TRIM29/1041)

It recognizes a 66kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29) . It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Product Specifications

Product Name Alternative

TRIM29||ATDC

Gene Name

TRIM29

Gene ID

23650

UniProt

Q14134

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein

Target Antigen

TRIM29 (Lung Squamous Cell Carcinoma Marker)

Clonality

Monoclonal

Clone

TRIM29/1041

Applications

ELISA, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

ELISA (For coating, order Ab without BSA) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)