Monoclonal Antibody to EGFR (Epidermal Growth Factor Receptor) (31G7 + GFR1195)

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-alphaa), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Specifications

Product Name Alternative

EGFR||ERBB||ERBB1||HER1

Gene Name

EGFR

Gene ID

1956

UniProt

P00533

Host

Mouse

Reactivity

Human

Immunogen

Human EGFR purified from A431 cells (31G7) ; Recombinant human EGFR protein (GFR1195)

Target Antigen

EGFR (Epidermal Growth Factor Receptor)

Clonality

Monoclonal

Clone

31G7 + GFR1195

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Digest formalin-fixed tissues with Protease (Sigma Cat. #P-6911) at 1mg/ml PBS, pH 7.4 for 10 min at 37C)