Monoclonal Antibody to EGFR (Epidermal Growth Factor Receptor) (Clone : SPM622)

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-alphaa), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Specifications

Product Name Alternative

EGFR||ERBB||ERBB1||HER1

Gene Name

EGFR

Gene ID

1956

UniProt

P00533

Host

Mouse

Reactivity

Human

Immunogen

Human EGFR purified from A431 cells

Target Antigen

EGFR (Epidermal Growth Factor Receptor)

Clonality

Monoclonal

Clone

SPM622

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Digest formalin-fixed tissues with Protease (Sigma Cat. #P-6911) at 1mg/ml PBS, pH 7.4 for 10 min at 37C)