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Monoclonal Antibody to AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) (Clone : C3)

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop) . This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Product Specifications

Product Name Alternative

AFP||HPAFP

Gene Name

AFP

Gene ID

174

UniProt

P02771

Host

Mouse

Reactivity

Human

Immunogen

Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Target Antigen

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)

Clonality

Monoclonal

Clone

C3

Applications

IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Isotype

Mouse IgG2a, kappa

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