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Polyclonal Antibody to GAPDH

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Product Specifications

Product Name Alternative

G3PD|| GAPD|| MGC88685

Gene Name

GAPDH

Gene ID

2597

UniProt

P04406

Host

Rabbit

Reactivity

Rat, Mouse, Human

Immunogen

Peptide sequence around aa.252~256 (P-A-K-Y-D) derived from Human GAPDH.

Target Antigen

GAPDH

Clonality

Polyclonal

Applications

WB

Format

Purified

Components

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Predicted MW: 37kd, Western blotting: 1:5000~1:10000

Isotype

Rabbit IgG

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