Polyclonal Antibody to Cyclin B1 (Ab-147)
The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
Product Specifications
Product Name Alternative
CCNB1
Gene Name
CCNB1
Gene ID
891
UniProt
P14635
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1.
Target Antigen
Cyclin B1 (Ab-147)
Clonality
Polyclonal
Applications
WB, IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
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