Polyclonal Antibody to OCT4
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency Pesce, M. and Scholer, H.R. (2001) Stem Cells 19, 271-278. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
Product Specifications
Product Name Alternative
Otc3|| OTF3|| OTF4|| OTF-3
Gene Name
POU5F1
Gene ID
5460
UniProt
Q01860
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4
Target Antigen
OCT4
Clonality
Polyclonal
Applications
WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 45kd, Western blotting: 1:1000
Isotype
Rabbit IgG
Frequently Asked Questions
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