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Polyclonal Antibody to eIF4E (Ab-209)

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Product Specifications

Product Name Alternative

MRNA cap-binding protein|| eIF-4F 25 kDa subunit

Gene Name

EIF4E

Gene ID

1977

UniProt

P06730

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.

Target Antigen

EIF4E (Ab-209)

Clonality

Polyclonal

Applications

WB, IHC, IF

Format

Purified

Components

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Predicted MW: 25kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Isotype

Rabbit IgG

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