Polyclonal Antibody to Chk2 (Ab-516)
The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541
Product Specifications
Product Name Alternative
CHEK2|| RAD53
Gene Name
CHEK2
Gene ID
11200
UniProt
O96017
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around aa. 514~518 (Q-P-S-T-S) derived from Human Chk2.
Target Antigen
Chk2 (Ab-516)
Clonality
Polyclonal
Applications
WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 62kd, Western blotting: 1:500~1:1000
Isotype
Rabbit IgG
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