Polyclonal Antibody to Bub3 (Phospho-Tyr207)
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Product Specifications
Product Name Alternative
Mitotic checkpoint protein BUB3||Hbub3||BUB3L||BUB3 budding uninhibited by benzimidazoles 3 homolog||budding uninhibited by benomyl
Gene Name
BUB3
Gene ID
9184
UniProt
O43684
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y (p) -L-D) derived from Human Bub3.
Target Antigen
Bub3 (Phospho-Tyr207)
Clonality
Polyclonal
Applications
WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blotting: 1:1000
Isotype
Rabbit IgG
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