Polyclonal Antibody to Histone H3 (Tri-Methyl-Lys27)
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Product Specifications
Product Name Alternative
H3/a||H3/c||H3/d||H3/f||H3/h
Gene Name
HIST1H3A
Gene ID
8350
UniProt
P68431
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around Tri-Methylation site of lysine 27 (A-R-K (tri-methyl) -S-A) derived from Human Histone H3.
Target Antigen
Histone H3 (Tri-Methyl-Lys27)
Clonality
Polyclonal
Applications
WB, IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 17kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
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