Polyclonal Antibody to Cdc25B (Phospho-Ser149)
Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. Li G, et al. (2010) Retrovirology.7:59. Wang Z, et al. (2010) BMC Cancer.10:233. Lucci MA, et al. (2010) Cell Oncol.32 (5-6) :361-72.
Product Specifications
Product Name Alternative
CDC25B
UniProt
Q9DB8
Host
Rabbit
Reactivity
Mouse
Immunogen
Peptide sequence around phosphorylation site of Serine 149 (F-R-S (p) -L-P) derived from Mouse Cdc25B.
Target Antigen
Cdc25B (Phospho-Ser149)
Clonality
Polyclonal
Applications
WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 62kd, Western blotting: 1:500~1:1000
Isotype
Rabbit IgG
Frequently Asked Questions
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