Polyclonal Antibody to FAK (phospho-Tyr576/Tyr577)
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613 Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688. Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155. Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
Product Specifications
Product Name Alternative
FADK 1|| FAK1|| PTK2
Gene Name
PTK2
Gene ID
5747
UniProt
Q05397
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y (p) -Y (p) -K-A) derived from Human FAK.
Target Antigen
FAK (phospho-Tyr576/Tyr577)
Clonality
Polyclonal
Applications
WB, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 125kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
Frequently Asked Questions
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